Fluorescence-based detection of ferrous iron in Senescent Cells
The lack of reliable biomarkers is a major limitation to aging research. They are unable to monitor the response to antiaging interventions or assess phenotypic change with age. This study examines intracellular ferrous (Fe2+), as a possible biomarker for senescence. Recent studies have shown that iron levels in senescent tissues increase dramatically. Current methods for measuring iron accumulation are inefficient and measure only total iron, not specific isotopes like the redox-active Fe2+. The damaging form of Iron (Fe2+), which is a toxic metal, is not elevated in senescent cell. In this study we evaluated the potential of using a newly developed Fe2+ reactive Probe (SiRhoNox-1), for selective labeling senescent cell in vitro. To achieve this, we generated different senescent cells models and labeled them with SiRhoNox-1. Our results show that SiRhoNox-1 selectively labels live senescent cell and is more specific and quicker than other staining methods such as SA-bGal and a fluorescent probe derived from C12 FDG. These findings suggest that SiRhoNox-1 could be used to detect senescent cellular based on ferrous iron levels.
Keywords: SiRhoNox-1; aging; biomarker; iron; senescence.
Source:
https://pubmed.ncbi.nlm.nih.gov/34841899/
