Exosomes from human pluripotent cells are subjected to a systemic proteomics analysis and miRNA profiling.
Exosomes derived from mesenchymal cells (MSCs) have been shown to be effective in treating a variety of diseases. They contain both protein and miRNA. The proteomics and microRNA profiles of the exosomes that are derived from human embryonic and pluripotent cells (hiPSCs and hESCs) are still unclear.
In this study we isolated exosomes using a classic ultracentrifugation method and 0.22-mm filters, followed by conservative identification. Tandem mass labeling and relative peptide quantification were used to define their proteomics. To determine miRNA profiles, high-throughput sequences were performed. We then performed a bioinformatics study to identify the biological pathways and processes modulated by exosomes. The western blot as well as RT-qPCR was performed to determine the exact amounts of miRNAs and proteins in the three types of exosomes.
According to our research, cargos from exosomes of three different types contribute to complex biological processes. Comparatively, hESC (hESC Exos), which regulate development, metabolism and anti-aging better than hiPSC (hiPSC Exos), and hiPSC (hiPSC Exos), which have similar biological functions to hESC (Exos), while hUC MSCs (hUCMSC-Exos), contribute more to immune regulation.
Source:
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03142-1
